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目的 :观察应用外源性碱性成纤维细胞生长因子 (b FGF)对大鼠肠缺血再灌注后肝功能与肠组织 P物质 (SP)含量的影响及其与组织损伤修复的关系。方法 :5 4只 Wistar大鼠随机分为 3组 :假手术组、生理盐水对照组及 b FGF治疗组 (每只大鼠 4μg b FGF)。生理盐水对照组及 b FGF治疗组动物于再灌注后 2、6、2 4和 48小时活杀 ,检测小肠组织 SP含量及血浆 D乳酸、二胺氧化酶 (DAO)和丙氨酸转氨酶 (AL T)水平。结果 :实验结果发现 b FGF治疗组血浆 AL T明显低于生理盐水对照组。 2组动物血浆 DAO于再灌注后 2～ 6小时均显著升高 ,b FGF治疗组 6小时血浆 DAO升高幅度更大 ,但 2 4小时均已恢复正常。生理盐水对照组动物伤后 6～ 48小时小肠组织 SP含量显著增加 ,血浆 D 乳酸与 SP呈平行性升高。而 b FGF治疗组动物小肠 SP及血浆 D 乳酸则无明显变化。结论 :缺血再灌注损伤后早期应用 b FGF可减轻肝功能的损伤 ,可能加重肠上皮的损伤 ,但后期抑制小肠 SP过量释放可能有利于肠道损伤的修复。
OBJECTIVE: To observe the effect of exogenous basic fibroblast growth factor (b FGF) on liver function and substance P content (SP) after intestinal ischemia-reperfusion in rats and its relationship with tissue injury repair. Methods: Forty five Wistar rats were randomly divided into three groups: sham operation group, saline control group and b FGF treatment group (4μg b FGF per rat). The rats in control group and b FGF group were sacrificed at 2, 6, 2, 4 and 48 hours after reperfusion. The content of SP in small intestine and the contents of plasma D-lactate, diamine oxidase (DAO) and alanine aminotransferase T) level. Results: The results showed that plasma ALT in bFGF treatment group was significantly lower than that in saline control group. DAO in 2 groups of animals increased significantly 2 ~ 6 hours after reperfusion, and the level of DAO in 6 hours of bFGF group increased more, but returned to normal after 24 hours. Compared with the control group, the content of SP in small intestine increased significantly in 6-48 hours after injury, and the plasma D-lactate increased in parallel with SP. The b FGF treatment group, animal intestine SP and plasma D lactic acid showed no significant change. CONCLUSION: Early application of bFGF after ischemia-reperfusion injury may reduce the damage of liver function, which may aggravate the injury of intestinal epithelium. However, inhibiting the excessive release of small intestine SP in the late stage may be beneficial to the repair of intestinal injury.