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【目的】观察紫茉莉根对前列腺增生(BPH)大鼠前列腺组织Ki67、CD34抗原表达的影响。【方法】选用SD大鼠,随机分为正常对照组,增生模型组,阴性对照组(灌胃生理盐水),紫茉莉根低、高剂量组(剂量分别为30、150 g.kg-1.d-1),非那雄胺组(剂量为1 mg.kg-1.d-1),除正常对照组外,其他组大鼠均皮下注射丙酸睾丸素(剂量为5 mg/kg,连续21 d)复制BPH模型;造模结束后,各组分别按设计剂量给药,连续30 d。第51天处死大鼠取各组大鼠前列腺进行组织病理检查,采用免疫组化法检测各组大鼠前列腺组织Ki67、CD34抗原表达。【结果】病理检查结果显示:增生模型组和阴性对照组前列腺组织细胞增生活跃,紫茉莉根高、低剂量组与非那雄胺组均可一定程度改善前列腺细胞增生的病理变化。增生模型组与阴性对照组Ki67与CD34表达水平升高,与正常对照组比较差异有显著性意义(P<0.01);紫茉莉根高、低剂量组与非那雄胺组均可降低Ki67、CD34表达水平,与增生模型组和阴性对照组比较差异有显著性意义(P<0.01),而3个给药组间比较差异无显著性意义(P>0.05)。【结论】紫茉莉根抗BPH的作用与降低前列腺组织Ki67、CD34抗原表达水平,抑制前列腺细胞增殖和新生血管产生有关。
【Objective】 To observe the effect of Mirabilis jalapa on the expression of Ki67 and CD34 antigen in prostatic tissue of benign prostatic hyperplasia (BPH) rats. 【Methods】 SD rats were randomly divided into normal control group, hyperplastic model group, negative control group (intragastric administration of normal saline) and Mirabilis jalapa root low and high dose groups (doses of 30 and 150 g · kg -1, respectively). d-1) and finasteride (1 mg.kg-1.d-1). Rats in other groups were given testosterone propionate (5 mg / kg, For 21 days, the BPH model was duplicated. After modeling, the rats were dosed by designed dose for 30 days. On the 51st day, the rats were sacrificed and the prostate of each group was taken for histopathological examination. Immunohistochemistry was used to detect the expression of Ki67 and CD34 in prostatic tissue. 【Results】 The results of pathological examination showed that prostatic hyperplasia in hyperplastic model group and negative control group were active. The root hyperplasia of Mirabilis jalapa, low dose group and finasteride group could all improve the pathological changes of prostatic cell proliferation. The expression of Ki67 and CD34 in hyperplastic model group and negative control group were significantly higher than those in normal control group (P <0.01), and Ki67 in root high, low dose and finasteride groups could decrease Ki67, (P <0.01). There was no significant difference between the three administration groups (P> 0.05). 【Conclusion】 The anti-BPH activity of Mirabilis jalapa is related to the decrease of the expression of Ki67 and CD34 antigen in prostate tissue and the inhibition of prostatic cell proliferation and neovascularization.