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目的:定量检测转化生长因子-(受体Ⅰ(transforming growth factor-(typeⅠreceptor,TβRⅠ)和受体Ⅱ(TβRⅡ)基因在大鼠视网膜中的表达水平,探讨TGF-(不同受体在视网膜中表达的差异及其意义。方法:分离取出大鼠视网膜,抽提RNA并逆转录,实时荧光定量PCR技术分析视网膜中TβRⅠ和TβRⅡ的mRNA含量。结果:TβRⅠ相对于18S的mRNA含量是0.00034±0·00013,TβRⅡ相对于18S的mRNA含量是0.0001±0·00005,差异有统计学意义(P<0.01)。在大鼠视网膜中以TβRⅠ表达为主,TβRⅠ和TβRⅡ比值的平均值为3·9±1.7。结论:实时荧光定量PCR技术能够针对性地精确分析极少量组织细胞的基因表达,TβRⅠ的mRNA在视网膜中的表达明显高于TβRⅡ,提示这可能是与TβRⅠ及TβRⅡ本身结构特点和在TGF-β信号转导过程中的不同作用有关。当TβRⅠ/TβRⅡ比例改变时,可影响细胞对TGF-β的应答反应,可能是增殖性视网膜病变的发生机制之一。
OBJECTIVE: To quantitatively detect the expression of transforming growth factor- (typeⅠreceptor (TβRⅠ) and receptor Ⅱ (TβRⅡ) in the rat retina and to explore the role of TGF- (different receptors in the retina) .Methods: The retinas of rats were isolated and extracted, RNA was extracted and reverse transcribed, and the mRNA levels of TβRⅠ and TβRⅡin the retina were analyzed by real-time fluorescence quantitative PCR.Results: The mRNA level of TβRⅠin 18S was 0.00034 ± 0 · 00013.The mRNA level of TβRⅡ relative to 18S was 0.0001 ± 0.00 00005, the difference was statistically significant (P <0.01) .The expression of TβRⅠ in the retina of rats was dominant, the average ratio of TβRⅠ to TβRⅡ was 3. 9 ± 1.7. Conclusion: Real-time PCR can accurately and precisely analyze the gene expression of a very small amount of tissue cells. The mRNA expression of TβRI in the retina is significantly higher than that of TβRII, suggesting that this may be related to the structural features of TβRⅠand TβRII and the expression of TGFβRⅠ in TGF -β signal transduction.When the ratio of TβRⅠ / TβRⅡ is changed, it can affect the response of cells to TGF-β, which may be the mechanism of proliferative retinopathy One of the system.