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目的:采用紫外波长切换高效液相色谱法,建立同时测定壮骨关节丸中7种有效成分含量的方法。方法:色谱柱ZorbaxSB-C_(18)(4.6 mm×250 mm,5μm);流动相0.05%甲酸水溶液-乙腈,梯度洗脱;切换时间和检测波长:0～25 min,283 nm;25～30 min,365 nm;30～32 min,270 nm;32～37 min,246 nm;37～65 min,322 nm;柱温:40℃;流速:1.0 mL·min~(-1)。结果:柚皮苷、槲皮素、淫羊藿苷、补骨脂素、异补骨脂素、芒柄花素和蛇床子素在相应的浓度范围内与峰面积呈良好线性关系,相关系数均大于0.9997;平均回收率(n=6)在97.8%～104.3%之间,RSD均小于4.0%。结论:本方法简便快速、准确、精密度高、重复性好,适用于壮骨关节丸的质量控制。
Objective: To establish a method for simultaneous determination of 7 active ingredients in Zhuanggu Pill by UV-wavelength-switching high performance liquid chromatography. Method: Chromatographic column ZorbaxSB-C_(18)(4.6 mm×250 mm, 5μm); mobile phase 0.05% formic acid aqueous solution-acetonitrile, gradient elution; switching time and detection wavelength: 0～25 min, 283 nm; 25～30 Min, 365 nm; 30-32 min, 270 nm; 32-37 min, 246 nm; 37-65 min, 322 nm; column temperature: 40°C; flow rate: 1.0 mL·min-1. Results: There was a good linear relationship between naringin, quercetin, icariin, psoralen, isopsoralen, formononetin, and osthole in the corresponding concentration range. All were greater than 0.9997; the average recovery (n=6) was between 97.8% and 104.3%, and RSD was less than 4.0%. Conclusion: This method is simple, rapid, accurate, high precision, and reproducible. It is suitable for the quality control of Zhuanggu joint pills.