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目的:通过90天喂养试验观察冬凌草的亚慢性毒性,为进一步开发冬凌草作为保健食品提供科学依据。方法:取体重60 g～90 g的SD大鼠80只,随机分为对照组和冬凌草低、中、高剂量组(1.667 g/kg.bw、3.333 g/kg.bw、5.000 g/kg.bw),每组20只,雌雄各半。按2.083%、4.167%、6.250%比例将冬凌草粉末掺入饲料中喂饲动物,对照组喂饲基础饲料,连续90天。试验期间每周加食2～3次,称一次体重,计算食物利用率;实验中期和末期检查大鼠血常规、生化指标;试验末取大鼠主要脏器作组织病理学检查。结果:90天喂养试验显示冬凌草粉末对大鼠各项观察指标未产生明显毒副作用,其最大无作用剂量为5.000 g/kg.bw。结论:90天喂养试验提示在本实验条件下冬凌草对机体基本安全。
Objective: To observe the subchronic toxicity of Rabdosia rubescens through a 90-day feeding experiment, and provide scientific basis for further development of Rabdosia rubra as a health food. METHODS: Eighty Sprague-Dawley rats weighing 60 g to 90 g were randomly divided into control group and low-, medium-, and high-dose groups of R. dybowskii (1.667 g/kg.bw, 3.333 g/kg.bw, and 5.000 g/g). Kg.bw), 20 in each group, male and female. The poplar powder was incorporated into the feed at a ratio of 2.083%, 4.167%, and 6.250%, and the control group was fed with the basic feed for 90 consecutive days. During the experimental period, they were fed 2 to 3 times a week to weigh one body weight, and the food utilization rate was calculated. The blood routine and biochemical indexes of the rats were examined during the middle and end of the experiment. The main organ of the rats was taken for histopathological examination at the end of the experiment. RESULTS: The 90-day feeding experiment showed that the rabdosia powder did not produce obvious toxic side effects on the various indexes of the rats, and the maximum no-effect dose was 5.000 g/kg.bw. Conclusion: The 90-day feeding test suggested that Rabdosia rubescens is basically safe to the organism under the experimental conditions.