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炸荚是大豆的一种自然特征属性,是影响大豆产量的重要因素之一。本研究以前期获得的转大豆炸荚相关基因GmAGL8的T_1代植株为材料,继续繁育获得T_2和T_3代;采用PCR和RT-q PCR检测方法对转基因植株进行基因遗传稳定性和表达情况分析;并以野生型中黄10号为对照,对大豆炸荚性状进行了鉴定分析。结果表明:转基因植株阳性率T_1代为87.5%,T_2和T_3代均达到100%,说明GmAGL8基因已基本能够在转基因后代中稳定遗传。RT-q PCR检测结果显示,转基因植株中GmAGL8基因的相对表达量都明显高于非转基因植株,且各转基因植株之间表达量具有差异性。对T_1、T_2和T_3代植株炸荚率进行了统计,不同世代转基因大豆的平均炸荚率为9.09%,而非转基因大豆炸荚率为83.3%,转基因与非转基因大豆之间炸荚率存在显著差异。综上所述,GmAGL8基因已基本实现在大豆转基因后代中稳定遗传并正常表达,表型鉴定结果初步证明了GmAGL8基因与大豆炸荚性状相关。
Deep-fried pods are a natural characteristic of soybean and are one of the important factors affecting the yield of soybean. In this study, T_1 generation of GmAGL8 gene, which was transferred to soybean blast pod, was used as material to continue breeding for T_2 and T_3 generation. The genetic stability and expression of transgenic plants were analyzed by PCR and RT-q PCR. The wild type Zhonghuang 10 as a control, soybean pod traits were identified and analyzed. The results showed that the positive rate of transgenic plants was 87.5% in T 1 generation and 100% in T 2 and T 3 generation, which indicated that GmAGL8 gene could be inherited stably in transgenic progenies. RT-q PCR results showed that the relative expression level of GmAGL8 gene in transgenic plants was significantly higher than that in non-transgenic plants, and the expression level of each transgenic plant was different. The rate of fried pods in T_1, T_2 and T_3 plants was calculated. The average rate of fried pods in different generation of transgenic soybean was 9.09%, while the rate of non-transgenic soybean fried pods was 83.3%. The ratio of fried pods among transgenic and non-transgenic soybean was Significant differences. In summary, GmAGL8 gene has been basically inherited and expressed normally in the progeny of soybean. The phenotypic identification proves that the GmAGL8 gene is related to the character of soybean pod.