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在感染期幼虫恢复进食的研究中,于模拟宿主条件下,水中保存的幼虫发生的反应明显低于相同条件以有粪培养基保存的幼虫,表明适宜的培养条件更能保持对宿主信号的应答。可以解释为因保存液溶质浓度不同所引起。以粪培养的钩虫三期幼虫经蒸馏水洗3次,然后用抗菌素水溶液将虫制成悬液,放在培养皿中于25℃孵育过夜,然后等分为3组,分别用有灭菌蒸馏水,pH6.8灭菌缓冲盐水及抗菌素溶液的培养皿中孵育,以有抗
In the study of infestation of larvae during infestation, the responses of larvae preserved in water under simulated host conditions were significantly lower than those of larvae maintained in the same conditions with fecal medium, indicating that appropriate culture conditions are more responsive to host signals . This can be explained by differences in the concentration of solutes in the preservation solution. The third stage larvae of hookworm cultured in feces were washed three times with distilled water and then the suspension was made into suspension with antibiotic aqueous solution and incubated in a petri dish at 25 ° C overnight and then equally divided into three groups with sterilized distilled water, pH6.8 sterile buffered saline and antibiotic solution in a petri dish incubated with anti-