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分别用含0.01％的芸香甙、槲皮素和2—十三烷酮的人工饲料连续饲养棉铃虫Helicoverpa armigera H(?)bner 1～7代,测定不同处理的棉铃虫种群谷胱甘肽S—转移酶(GST)和乙酰胆碱酯酶(AChE)的活性变化以及幼虫对杀虫剂的反应,以单独人工饲料饲养的种群作为对照。芸香甙F_1代对甲基对硫磷的耐药性提高3倍,槲皮素F_1、F_2代和2—十三烷酮F_1代对甲基对硫磷的耐药性均有所提高,对灭多威和溴氰菊酯的耐药性则变化不大。芸香甙F_1代GST活性是对照的57倍。各处理种群GST的活性和AChE对底物的亲和力都随着棉铃虫取食世代的增加而提高。但是,AChE对对氧磷的敏感度也在不断提高。说明植物次生物质对棉铃虫耐药性的影响是解毒酶和靶标酶改变的综合结果。
Helicoverpa armigera H (?) Bner was inoculated continuously for 1 to 7 generations with artificial feed containing 0.01% rutin, quercetin and 2-tridecanone respectively to determine the effects of different treatments on the population of glutathione S - Changes in the activity of transfected enzyme (GST) and acetylcholinesterase (AChE) as well as larvae response to insecticides, with a population fed on artificial diet alone as a control. Rutin glycosides F_1 on methyl parathion resistance increased by 3 times, quercetin F_1, F_2 generation and 2-tridecanone F_1 methyl parathion resistance were increased, the The resistance of methomyl and deltamethrin changed little. Rutin glycosides F1 generation GST activity is 57 times the control. The activity of GST and the affinity of AChE to substrates in all treatment populations increased with the increase of feeding generations of cotton bollworm. However, AChE is also increasingly sensitive to paraoxon. The effect of plant secondary metabolites on the resistance of H. armigera was a combined result of the changes of detoxification enzymes and target enzymes.