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1990年以来,作者应用电镜技术、病毒分离提纯技术对云南的烟草病毒病样品进行鉴定研究,同时,对重病区宾川进行集中采样调查及防治试验,结果报道如下。一、材料与方法 (一)材料烟草病毒样品采自玉溪、澄江等12个县市烤烟生产田。PVX、PVY标准抗原与抗体由中国农业科学院生物技术中心提供。TMV、CMV、PVY标样电镜图谱由北京农业大学电镜室、病毒室提供。 (二)方法按采集地点、栽培品种、症状类型等分类采样,参照陈保善等(1986)、田波等(1987)及Hamilton等(1981)的方法制样。样品置JEM100CX—Ⅲ型透射电子显微镜下放大5万倍观察30个以上有效视野,检测病原种类及其相对含量、带毒率,研究分布特点。用NS—83增抗剂、病毒A及硫酸锌作防治处理,清水作对照,考察对病情的抑制效果。
Since 1990, the author applied electron microscopy and virus isolation and purification technology to identify the samples of tobacco virus disease in Yunnan Province. At the same time, the authors conducted a centralized sampling survey and prevention and control test on Binchuan in the seriously ward area. The results are reported as follows. I. Materials and Methods (I) Materials The samples of tobacco virus were collected from flue-cured tobacco production fields in 12 counties such as Yuxi and Chengjiang. PVX, PVY standard antigens and antibodies provided by the Chinese Academy of Agricultural Sciences Biotechnology Center. TMV, CMV, PVY standard electron microscopy by the Beijing Agricultural University, electron microscope room, virus room. (2) Methods Sampling was conducted according to the sampling sites, cultivars and types of symptoms, with reference to Chen Baoshan et al. (1986), Tian Bo et al. (1987) and Hamilton et al. (1981). Samples were placed JEM100CX-Ⅲ under transmission electron microscope magnification of 50,000 times to observe more than 30 effective field of vision to detect the pathogen species and their relative content, the rate of poisoning, study distribution characteristics. With NS-83 enhancers, virus A and zinc sulfate for prevention and treatment, water as a control to examine the effect of the disease.