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绒毛(CV)标本直接制备染色体通常有很少的分裂相,但过夜培养后可得到较好结果,说明绒毛经活检损伤和送到实验室后需要时间恢复。用特殊抗体染色技术了解胸苷类似物的掺入,测定BrdU摄入量,从而监测绒毛的增殖动力学。绒毛标本取自流产物,在含5%FCS的Chang氏培养液中培养。BrdU的用法有两种:(1)活检后直接用BrdU(0.03mM)连续处理,或在24小时后,收获前处理2～5小时;(2)活检后将等份标本按0、3、6和24小时加入BrdU(0.03mM)处理1小时,通过换液和过量胸苷(0.3mM)终止BrdU的作用。6和8小时后收获细胞,以保证至少已通过部分S期。免疫细胞
Chromosomes usually have very few cleaved phases for direct preparation of chromosomes, but good results are obtained after overnight culture, indicating that villi require time to recover from biopsy damage and delivery to the laboratory. The incorporation of thymidine analogues was measured using special antibody staining techniques and BrdU uptake was measured to monitor the kinetics of villus proliferation. Villus specimens were taken from the flow product and cultured in Chang’s medium with 5% FCS. BrdU is used in two ways: (1) biopsies are treated directly with BrdU (0.03 mM), or 24 hours before harvesting for 2 to 5 hours; (2) BrdU (0.03 mM) was added for 1 hour at 6 and 24 hours, and the effect of BrdU was stopped by changing the fluid and excess thymidine (0.3 mM). Cells were harvested after 6 and 8 hours to ensure that at least part of the S phase had passed. Immune Cells