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研究了人淋巴因子ｒｈＩＬ－４、ｒｈＩＬ－２、ｒｈＩＦＮ－γ对ＰＭＡ活化的扁桃体Ｂ淋巴细胞ＣＤ２３分子表达的调节作用。实验结果表明：１．ｒｈＩＬ－４能够显著增加ＰＭＡ作用下的Ｂ细胞ＣＤ２３表达，呈剂量依赖性反应；ｒｈＩＦＮ－γ对活化后Ｂ细胞ＣＤ２３表达呈明显抑制作用；ｒｈＩＬ－２对ＣＤ２３表达的抑制作用微弱。２．在ＰＭＡ单独刺激下Ｂ淋巴细胞ＣＤ２３的表达在第３天达高峰，而ＰＭＡ与ＩＬ－４共同作用下ＩＬ－４能够持续促进ＰＭＡ作用下的扁桃体Ｂ细胞ＣＤ２３的高表达，在作用的第５天ＣＤ２３阳性率可高达７５．４％。由此可见Ｂ细胞ＣＤ２３分子的表达受到Ｔ细胞源性淋巴因子的调控，这种调控对于免疫应答的调节可能具有重要意义。此外对Ｂ淋巴细胞经ＰＭＡ及淋巴因子活化后，反映细胞分裂状态的￣３Ｈ－ＴｄＲ、￣３Ｈ－ＵＲ掺入ｃｐｍ值与ＣＤ２３表达的相关性进行探索。发现两者无明显相关性。
The regulatory effect of human lymphokines rhIL-4, rhIL-2 and rhIFN-γ on CD23 expression in PMA-activated tonsil B lymphocytes was studied. The experimental results show that: 1. rhIL-4 could significantly increase CD23 expression of B cells under the action of PMA in a dose-dependent manner; rhIFN-γ significantly inhibited the expression of CD23 on activated B cells; and rhIL-2 had a weak inhibitory effect on CD23 expression. 2. The expression of CD23 on B lymphocytes reached the peak on the 3rd day under the stimulation of PMA alone, while the combination of PMA and IL-4 could continuously promote the high expression of CD23 on tonsillar B cells under the action of PMA. CD23 positive rate of up to 75.4%. This shows that the expression of CD23 molecules on B cells is regulated by T-cell-derived lymphokines, and this regulation may be of great significance for the regulation of immune responses. In addition, the B lymphocyte activation by PMA and lymphocytes, reflecting the state of cell division ~ 3H-TdR, ~ 3H-UR incorporation cpm value and CD23 expression was explored. Found no significant correlation between the two.