论文部分内容阅读
[目的]探讨续断提取物对血管性痴呆模型大鼠学习记忆能力的影响.[方法]取雄性SD大鼠,随机分为正常对照组、假手术组、模型对照组和续断提取物高、中、低剂量(13.5,4.5,1.5g/L)组.采用反复夹闭、再通双侧颈总动脉同时腹腔注射给予硝普钠方法建立大鼠血管性痴呆模型.模型建立成功后灌胃给予各给药组相应剂量的药物,每日1次,连续28d.利用Morris水迷宫试验检测各组大鼠学习记忆能力;取心脏血测定超氧化物歧化酶(SOD)、丙二醛(MDA)及谷胱甘肽过氧化物酶(GSH-Px)水平.[结果]与正常对照组和假手术组比较,模型对照组寻找站台潜伏期明显延长(P<0.05),撤出平台后穿越平台次数明显减少(P<0.05),血清中MDA含量明显升高(P<0.05),SOD及GSH-Px水平明显降低(P<0.05).与模型对照组比较,续断提取物中、高剂量组寻找站台潜伏期明显缩短,血清中MDA含量明显降低(P<0.05),SOD及GSH-Px水平明显升高(P<0.05),穿越平台次数明显增加(P<0.05).[结论]续断提取物可改善血管性痴呆模型大鼠学习记忆能力.
[Objective] To explore the effect of Dipsacus sepculata extract on learning and memory abilities of vascular dementia model rats. [Methods] Male SD rats were randomly divided into normal control group, sham operation group, model control group, , Low dose (13.5,4.5,1.5g / L) group.Using repeated clipping, then bilateral common carotid artery and intraperitoneal injection of sodium nitroprusside method to establish rat vascular dementia model.Model establishment after successful irrigation The rats in the stomach were given the corresponding doses of drugs, once a day for 28 days. Morris water maze test was used to detect the learning and memory abilities of rats in each group. The cardiac blood was taken for determination of superoxide dismutase (SOD), malondialdehyde MDA and GSH-Px. [Results] Compared with the normal control group and the sham operation group, the latency of the model control group was significantly longer (P <0.05) (P <0.05), the content of MDA in serum increased significantly (P <0.05) and the level of SOD and GSH-Px decreased significantly (P <0.05). Compared with the model control group, In the dose group, the latent period of searching platform was shortened obviously, the content of MDA in serum was significantly decreased (P <0.05), the level of SOD and GSH-Px Increased (P <0.05), a significant increase in the number of internet through (P <0.05). [Conclusion] Dipsacus extract can improve learning and memory in rat vascular dementia model.