对脾虚证的实质进行深入的研究是一个重要课题。本实验应用体内参入~3氢-胸腺嘧啶核苷(~3H-TdR)的放射自显影方法,就脾虚证动物十二指肠粘膜上皮细胞的动力变化进行了探讨,胃肠道中上皮细胞迅速恒定更新的概念早已确立。30年前Leblond在小肠隐窝中计算了分裂率,导致了细胞从隐窝移行出来、大约在二天左右沿着绒毛壁向上秽行到顶端的观点,并应用注入脱氧核糖核酸的前身物的放射自显影术,使之成为可见的过程。 The in-depth study of the essence of spleen deficiency syndrome is an important issue. In this experiment, autoradiography of ~3H-TdR in vivo was used to investigate the dynamic changes of duodenal epithelial cells in spleen-deficiency animal. The epithelial cells in the gastrointestinal tract were rapidly constant. The concept of updating has long been established. Thirty years ago, Leblond calculated the division rate in the small intestine crypts, resulting in the migration of cells from the crypts, circling up to the top in about two days along the wall of the villi, and using the radiation injected into the precursor of the DNA. Autoradiography makes it a visible process.