论文部分内容阅读
目的探讨凋亡抑制基因Survivin在非小细胞肺癌(NSCLC)组织中的表达情况及其与预后的关系。方法100例NSCLC石蜡标本,应用免疫组织化学SP法及原位杂交方法检测Survivin在NSCLC组织中的表达情况。结果Survivin蛋白阳性表达率为72.0%,Survivin mRNA阳性表达率为76.0%。随TNM分期增高,Survivin蛋白及mRNA阳性表达率增高。淋巴结转移者较无转移者阳性表达率高。Survivin阳性表达与NSCLC组织学类型、分化程度无相关性。Survivin mRNA水平与其蛋白水平表达一致。结论NSCLC组织中Survivin mRNA与蛋白均高表达;Survivin蛋白水平和mRNA水平表达一致;Survivin阳性表达与TNM分期及淋巴结转移密切相关。
Objective To investigate the expression of survivin gene in non-small cell lung cancer (NSCLC) and its relationship with prognosis. Methods 100 cases of NSCLC paraffin specimens were examined for the expression of Survivin in NSCLC tissues by immunohistochemical SP method and in situ hybridization. Results The positive rate of Survivin protein was 72.0% and the positive rate of Survivin mRNA was 76.0%. With TNM staging, Survivin protein and mRNA positive expression rate increased. The positive rate of lymph node metastasis than those without metastasis was high. There was no correlation between the positive expression of Survivin and histological type and differentiation of NSCLC. Survivin mRNA level is consistent with its protein level. Conclusion Survivin mRNA and protein are both highly expressed in NSCLC. The expression of Survivin is consistent with that of mRNA. The positive expression of Survivin is closely related to TNM staging and lymph node metastasis.