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以引起牡丹柱枝孢叶斑病的加拿大柱枝双孢霉(Cylindrocladium canadense)为试材,采用菌丝生长速率法和菌丝干重法研究了3类8种杀菌剂对病菌菌丝生长的影响,并进一步采用插片法及染色法,研究了多菌灵、氟环唑及嘧菌酯对菌丝线性生长及菌落形态的影响。结果表明:苯并咪唑类、三唑类及甲氧基丙烯酸酯类杀菌剂对病菌菌丝的线性生长及菌丝干重均有一定的抑制作用。3类杀菌剂均导致菌丝顶端分枝数增多、间距缩短;杀菌剂处理的菌落边缘菌丝茂密,放射性生长的幼龄菌丝束较少,向外扩展速度慢。3类杀菌剂对病菌都表现为抑菌作用,均可作为柱枝孢叶斑病化学防治的备选药剂而应用在牡丹病害的综合防控中。
Cylindrocladium canadense, a species that causes spore leaf spot in Peony, was used as test material to study the mycelial growth rate of mycelia of three species of eight fungicides by mycelial growth rate and mycelial dry weight The effects of carbendazim, epoxiconazole and azoxystrobin on the linear growth and colony morphology of mycelium were also studied. The results showed that the benzimidazoles, triazoles and methoxyacrylates could inhibit the growth of mycelium and the mycelial dry weight of the mycelium. All three kinds of fungicides resulted in the increase of the number of branch branches at the top of the mycelium and the shortened spacing. The mycelia at the edges of the fungicide-treated colonies were dense, with fewer young fungal filaments growing radially and slow outward expansion. All three kinds of fungicides showed bacteriostasis to bacteria, and they could be used as an alternative medicament for chemoprevention of spore leaf spot disease in the integrated prevention and control of peony diseases.