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目的:建立同时测定灯银脑通胶囊中白果内酯、银杏内酯A、三七皂苷R1、人参皂苷Rg1含量的高效液相色谱-蒸发光散射检测法。方法:采用Diamonsil C18(200mm×4.6mm,5μm)色谱柱,流动相为甲醇-水-四氢呋喃(25∶70∶12),流速为1.0mL·min-1,柱温25℃,漂移管温度为110℃,载气流速为2.8L·min-1。结果:样品中白果内酯、银杏内酯A、三七皂苷R1、人参皂苷Rg1分离良好,线性范围分别为0.02～0.2mg·mL-1(r=0.9999),0.126～1.26mg·mL-1(r=0.9999),0.108～1.08mg·mL-1(r=0.9995),0.306～3.06mg·mL-1(r=0.9997);方法平均回收率(n=9)分别为100.0%,99.9%,99.8%,100.1%。结论:本法准确,专属性强,重现性好,可用于灯银脑通胶囊的质量控制。
Objective: To establish a method for simultaneous determination of ginkgolide, ginkgolide A, notoginsenoside R1 and ginsenoside Rg1 in Dengyinnaotong Capsule by high performance liquid chromatography-evaporative light scattering detection. Methods: A Diamonsil C18 (200mm×4.6mm, 5μm) column was used. The mobile phase was methanol-water-tetrahydrofuran (25∶70∶12), the flow rate was 1.0mL·min-1, the column temperature was 25°C, and the drift tube temperature was At 110°C, the carrier gas flow rate was 2.8 L·min-1. Results: The ginkgolide, ginkgo lactone A, notoginsenoside R1, and ginsenoside Rg1 were well separated in the samples with linear ranges of 0.02-0.2 mg·mL-1 (r=0.9999) and 0.126-1.26 mg·mL-1, respectively. (r=0.9999), 0.108 to 1.08 mg·mL-1 (r=0.9995), 0.306 to 3.06 mg·mL-1 (r=0.9997); the average recovery of the method (n=9) was 100.0% and 99.9%, respectively. , 99.8%, 100.1%. Conclusion: This method is accurate, specific and reproducible, and can be used for the quality control of Dengyinaotong Capsule.