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目的探讨实验性大肠癌对机体免疫功能的影响。方法利用1,2-二甲肼诱导大鼠建立大肠癌模型,流式细胞术检测成瘤16、21周外周血淋巴细胞和21周脾细胞中CD3、CD4、CD8、CD25、4-1BB和4-1 BBL表达率,ELISA法检测成瘤21周时脾细胞经植物血凝素(PHA)刺激后分泌γ干扰素(IFN-γ)的能力。结果 21周时的诱癌率达100%(5/5)。16周时成瘤组外周血淋巴细胞CD3~+和CD3~+ CD4~+表达率较对照组略有降低,但差异无统计学意义(P>0.05);21周时成瘤组外周血淋巴细胞和脾细胞中CD3~+ CD4~+表达率较对照组明显降低(P<0.05),4-1BB和4-1 BBL的表达较对照组均降低,但差异均无统计学意义(均P>0.05);成瘤组脾细胞PHA刺激试验后分泌IFN-γ的能力较对照组显著下降(P<0.01)。结论实验性大肠癌同人类大肠癌具有类似的免疫功能降低,主要是细胞免疫功能的下降。
Objective To investigate the effect of experimental colorectal cancer on immune function. Methods The rat model of colorectal cancer was induced by 1,2-dimethylhydrazine. The expressions of CD3, CD4, CD8, CD25 and 4-1BB in peripheral blood lymphocytes and 21-week-old spleen cells 4-1 BBL expression was detected by enzyme-linked immunosorbent assay (ELISA). The ability of IFN-γ secreted by splenocytes stimulated by phytohemagglutinin (PHA) was detected by ELISA at 21 weeks after tumorigenesis. The results of the 21-week cancer induction rate of 100% (5/5). The expression of CD3 ~ + and CD3 ~ + CD4 ~ + in peripheral blood lymphocytes decreased slightly at 16 weeks in the tumor-bearing group compared with the control group, but there was no significant difference (P> 0.05). At 21 weeks, the peripheral blood lymphocytes and splenocytes (P <0.05). The expression of CD3 ~ + CD4 ~ + in 4-1BB and 4-1 BBL groups was lower than that in control group (all P> 0.05). The ability of secreting IFN-γ secreted by PHA stimulating test in spleen cells of tumor-bearing group was significantly lower than that of control group (P <0.01). Conclusion Experimental colorectal cancer with human colorectal cancer has a similar reduction in immune function, mainly cellular immune function decline.