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目的：选育高产γ-亚麻酸被孢霉菌株。方法：以被孢霉（Mortierellasp．）为出发菌株经过UV诱变处理和甘草酸筛选，获得一株γ-亚麻酸（GLA）高产菌株A02变株。结果：变株摇瓶培养生物量达20．12g／L，较出发菌株提高25．50％，γ-亚麻酸的产量为0．96g／L，较出发菌株提高了81％。传代实验显示，变株A02具有良好的遗传稳定性。结论：甘草酸筛选法是多不饱和脂肪酸产生前育种工作的一个有效方法。
Objective: Breeding of high yield γ-linolenic acid Mortierella strains. Methods: A strain of strain A02 with high yield of γ-linolenic acid (GLA) was obtained from Mortierellasp. Strain after UV mutagenesis and glycyrrhizic acid screening. Results: The biomass of shake flask culture was 20.12g / L, which was 25.50% higher than that of the original strain. The yield of γ-linolenic acid was 0.96g / L, which was 81% higher than the original strain. Passage experiments showed that the mutant A02 has a good genetic stability. Conclusion: Glycyrrhizic acid screening method is an effective method for breeding of polyunsaturated fatty acids before breeding.