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目的:了解中国经采供血HIV感染长期不进展者CD4+T淋巴细胞趋化因子受体表达,分析其与疾病不进展的关系。方法:收集43例经采供血HIV感染长期不进展者、82例无症状HIV感染者、35例AIDS病人及40例健康对照的抗凝全血,用流式细胞仪检测趋化因子受体CCR5、CXCR4的表达,并分析其与病毒载量、CD4+T淋巴细胞绝对值及T淋巴细胞活化的相关性。结果:长期不进展组CD4+T细胞表面CCR5的表达明显低于无症状HIV感染组及AIDS组(P<0.01),与健康对照无显著差异;CD4+T细胞表面CXCR4的表达各组无显著差异。CD4+T细胞表面CCR5的表达与CD4+T细胞数量显著负相关(r=-0.498,P<0.05),与病毒载量无显著相关性。CD4+T细胞表面CCR5的表达与HLA、CD38在CD4+、CD8+T细胞的表达水平显著正相关(P<0.001,CD38在CD4+T细胞的表达除外),CD4+T细胞表面CXCR4的表达与HLA在CD4、CD8+T细胞的表达水平显著负相关(P<0.01)。结论:HIV感染长期不进展者CD4+T细胞趋化因子受体CCR5表达维持较低水平,与疾病不进展相关。
OBJECTIVE: To understand the expression of CD4 + T lymphocyte chemokine receptor in long-term non-progressing HIV infection in China and analyze its relationship with disease progression. Methods: A total of 43 anticoagulant whole blood samples from 43 HIV-infected donors, 82 asymptomatic HIV-infected patients, 35 AIDS patients and 40 healthy controls were collected. Flow cytometry was used to detect chemokine receptor CCR5 , CXCR4 expression, and analyze its correlation with viral load, the absolute value of CD4 + T lymphocytes and T lymphocyte activation. Results: The expression of CCR5 on the surface of CD4 + T cells in long-term progression group was significantly lower than that in asymptomatic HIV infection group and AIDS group (P <0.01), but no significant difference was found between the expression of CXCR4 and CD4 + T cells difference. The expression of CCR5 on CD4 + T cells was significantly and negatively correlated with the number of CD4 + T cells (r = -0.498, P <0.05), but not to the viral load. The expression of CCR5 on CD4 + T cells was positively correlated with the expression of HLA and CD38 on CD4 + and CD8 + T cells (P <0.001, except CD38 expression on CD4 + T cells), and the expression of CXCR4 on CD4 + T cells The expression of HLA in CD4 and CD8 + T cells was significantly and negatively correlated (P <0.01). Conclusion: The expression of CCR5, a CD4 + T cell chemokine receptor, is maintained at a low level for a long time without progressing HIV infection, which is related to the non-progression of the disease.