论文部分内容阅读
通过研究Ru(II)多吡啶配合物[Ru(bpy)2(pmip)]2+与DNA的相互作用的光谱变化,发现[Ru(bpy)2(pmip)]2+-DNA体系相互作用模式既存在插入模式,也存在静电吸引模式。同时基于该体系增强的共振光散射现象,建立了一种简单、快速的测定纳克级核酸的新方法。在最佳反应条件下,体系在373 nm处共振光散射强度的增强与DNA的浓度呈线性关系,线性范围为0.065～2.400 mg/L,线性公式为△I RLS=144.09c+7.44(mg/L),相关系数为0.9985,ctDNA的检出限为6.04 ng/mL。
The interaction of [Ru (bpy) 2 (pmip)] 2 + -DNA system was observed by studying the spectral changes in the interaction of Ru (II) polypyridine complex [Ru There are both insert modes and electrostatic attraction modes. At the same time, based on the enhanced resonance light scattering of the system, a simple and rapid method for the determination of nanokines was established. Under optimum reaction conditions, the enhancement of the light scattering intensity at 373 nm showed a linear relationship with the concentration of DNA, the linear range was 0.065-2.400 mg / L, the linear equation was △ I RLS = 144.09c + 7.44 (mg / L), the correlation coefficient was 0.9985, and the detection limit of ctDNA was 6.04 ng / mL.