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为探索野生枝瑚菌多糖水浸提的最佳条件,在单因素实验基础上,采用合理的实验设计方案,应用响应面分析法优化野生枝瑚菌多糖的提取条件,并采用柱前衍生高效液相色谱法测定野生枝瑚菌多糖的单糖组成。根据回归分析得到野生枝瑚菌粗多糖的最佳提取条件为温度91℃,水料比32∶1(m L/g),时间3.2 h,在此条件下野生枝瑚菌多糖得率为1.97%。高效液相色谱分析结果表明枝瑚菌多糖由甘露糖、核糖、葡萄糖、半乳糖、木糖、岩藻糖六种单糖组成,各组分摩尔比为:45.8∶10.6∶76.4∶8.53∶4.63∶5.24。实验结果表明,响应面法对野生枝瑚菌多糖的提取条件优化合理可行,PMP-HPLC法,可用于测定野生枝瑚菌多糖中单糖的组成。
In order to explore the optimal conditions for the water extraction of polysaccharides from wild coral fungi, a reasonable experimental design was used on the basis of single-factor experiments, and the extraction conditions of polysaccharides from wild coral fungi were optimized by response surface analysis. The pre-column derivatization Determination of Monosaccharide Compositions of Coptis Coli Polysaccharides by Liquid Chromatography. According to the regression analysis, the optimal extraction conditions for crude polysaccharides from Coral flora were as follows: temperature 91 ℃, ratio of water to material 32: 1 (m L / g) and time 3.2 h, the yield of wild Coptis polysaccharides was 1.97 %. High performance liquid chromatography (HPLC) analysis showed that the polysaccharides from Bercolin were composed of six monosaccharides, mannose, ribose, glucose, galactose, xylose and fucose. The molar ratio of each component was 45.8:10.6:76.4:8.53:4.63 : 5.24. The experimental results showed that the response surface methodology was reasonable and feasible to optimize the extraction conditions of polysaccharides from Bercidae, PMP-HPLC.