本文介绍一种新的微量光度技术——纸上吸光光度法。样品与显色剂反应,生成难溶配合物,后者由微量吸滤管收集在滤纸上,形成固定面积的圆形斑点,用721型分光光度计、双波长吸光度差值法定量测定。本方法灵敏度高,检出限可超过ppb级。精密度在1％～4％之间。应用这种方法测定了水中痕量 Ni(Ⅱ)、A g(Ⅰ) 和 F e(Ⅲ)。 This article describes a new micro-luminosity technique - on-paper absorptiometry. The sample reacts with the developer to form an insoluble complex, which is collected on the filter paper by a micropipette to form a fixed area of ​​circular spots, quantitatively determined by a 721 spectrophotometer with a two-wavelength absorbance difference method. The method of high sensitivity, detection limit can exceed ppb level. Precision between 1% to 4%. The trace Ni (Ⅱ), A g (Ⅰ) and Fe (Ⅲ) in water were measured by this method.