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目的建立人类血小板抗原(HPA)-15系统的分型方法,并应用于血小板供者库的HPA基因定型。方法采用本研究合成的引物及G&T公司的商品化试剂盒,应用PCR-SBT技术对200名随机的汉族血小板捐献者进行HPA-15系统基因分型,采用第l4届ISBT血小板协作组提供的l0份质控标本以及德国Inno-train公司提供的质控样品作为平行对照,进行验证。结果 200名汉族血小板志愿捐献者HPA基因频率为HPA-15a 0.430、HPA-15b 0.570;基因分型结果与ISBT公布的结果及Inno-train公司提供的质控样品的结果完全一致。结论所建立的HPA基因PCR-SBT分型技术具有高分辨率、高通量、高精确度、能直接发现新的等位基因等特点,具有广泛的应用前景。
Objective To establish a method of typing human HPA-15 system and apply it to HPA gene typing of platelet donor library. Methods 200 randomized Chinese platelet donors were genotyped by PCR-SBT using the primers and G & T commercial kit of the present study. The HPA-15 system was genotyped by the l4th ISBT platelet cooperation group Quality control samples and quality control samples provided by Germany Inno-train as a parallel control, to verify. Results The frequency of HPA gene in 200 Han volunteers was HPA-15a 0.430 and HPA-15b 0.570 respectively. Genotyping results were in good agreement with the results published by ISBT and the quality control samples provided by Inno-train. Conclusion The established HPA gene PCR-SBT typing technology has the characteristics of high resolution, high throughput, high accuracy and direct discovery of new alleles, and has broad application prospects.