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目的:通过检测分析2004年-2007年31株O139群霍乱弧菌临床分离株对13种抗菌药物耐药情况和相关耐药基因携带情况,为O139群霍乱弧菌预防和治疗提供理论依据。方法:K-B纸片扩散法药物敏感实验检测细菌13种抗菌药物耐药情况,用常规PCR方法扩增TEM、aadA2、strA、strB、tetA、floR和sul1等7种耐药基因和1型耐药整合子。结果:药物敏感实验表明31株O139群霍乱弧菌临床分离株只存在一种耐药谱型,对头孢噻肟、头孢吡肟、环丙沙星和氟哌酸敏感,对氨苄西林、羧苄西林、氯霉素、复方新诺明、四环素、强力霉素、庆大霉素、卡那霉素和链霉素耐药,均扩增出TEM、aadA2、strA、strB、floR和sul1耐药基因和1型耐药整合子结构。结论:O139群霍乱弧菌已对多种抗生素耐药并存在相关耐药基因,应引起临床重视。
OBJECTIVE: To provide a theoretical basis for the prevention and treatment of Vibrio cholerae O139 by detecting and analyzing the resistance of 13 strains of Vibrio cholerae O139 isolates from 2004 to 2007 to 13 antibacterials and the related resistance gene carriers. Methods: Thirty-three drug-resistant strains of bacteria were detected by KB disk diffusion method. The resistance genes of TEM, aadA2, strA, strB, tetA, floR and sul1 were amplified by conventional PCR. Integrator. Results: The results of drug susceptibility tests showed that there were only one type of drug resistance in 31 clinical isolates of Vibrio cholerae O139 isolates, which were sensitive to cefotaxime, cefepime, ciprofloxacin and norfloxacin, Xilin, chloramphenicol, cotrimoxazole, tetracycline, doxycycline, gentamicin, kanamycin and streptomycin were all resistant to TEM, aadA2, strA, strB, floR and sul1 Genes and Type 1 Drug-resistant Integron Structures. Conclusion: Vibrio cholerae O139 strains are resistant to many antibiotics and exist related resistance genes, which should arouse clinical importance.