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目的 探讨生长激素 (growthhormone,GH )对急性坏死性胰腺炎 (acutenecrotizingpancreatitis,ANP)肠道细菌移居的影响。方法 采用胆胰管内逆行注射 5 %牛磺胆酸钠溶液诱导大鼠ANP模型。 137只大鼠随机分成 3组 :假手术组 (n =41)、ANP +NS组 (n =48)和ANP +GH组 (n =48)。GH治疗组大鼠皮下注射 0 .75U/kg体重基因重组GH。观察血清淀粉酶、脂肪酶活性 ;血浆内毒素水平 ;脏器细菌培养 ;胰腺和肠黏膜病理学变化及大鼠存活率。结果 与ANP +NS组相比 ,GH显著降低血清淀粉酶、脂肪酶活性 ;减轻胰腺组织和肠黏膜炎症细胞浸润、水肿 ;降低血浆内毒素水平和脏器细菌培养阳性率 ;提高ANP大鼠存活率。结论 ANP大鼠存在肠道细菌移居 ;GH可减轻胰腺及肠道病理损伤、阻止肠道细菌移居 ,从而改善ANP大鼠预后
Objective To investigate the effect of growth hormone (GH) on intestinal bacterial colonization in patients with acute necrotizing pancreatitis (ANP). Methods ANP model was induced by retrograde injection of 5% sodium taurocholate into biliary and pancreatic ducts. 137 rats were randomly divided into 3 groups: sham operation group (n = 41), ANP + NS group (n = 48) and ANP + GH group (n = 48). GH treatment group rats subcutaneous injection of 0.75U / kg body weight gene recombinant GH. Serum amylase, lipase activity, plasma endotoxin level, bacterial culture of organ, pathological changes of pancreas and intestinal mucosa and survival rate of rats were observed. Results Compared with ANP + NS group, GH significantly decreased serum amylase and lipase activity, alleviated infiltration and edema of inflammatory cells in pancreatic tissue and intestinal mucosa, decreased plasma endotoxin levels and positive rates of bacterial culture in organs, and increased survival of ANP rats rate. Conclusions There is intestinal bacterial migration in ANP rats. GH can reduce pathological damage of pancreas and intestine and prevent intestinal bacteria from migrating, thus improving the prognosis of ANP rats