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曾报道制备柴胡抗溃疡果胶柴胡多糖2Ⅱc的活性部位分支(PG-1)的抗体(抗-柴胡多糖2Ⅱc/PG-1-IgG),用这种抗体探讨了柴胡的质量以及活性多糖的体内动态。推测这种抗体的抗原决定簇为对分支中的外-β(1→3)-半乳聚糖酶敏感的糖侧链部分。因此本次用各种糖苷酶进一步分析了分支的微细结构与抗体的抗原决定簇。
It has been reported that the antibody (anti-Bupleurum chinensis polysaccharide IIc/PG-1-IgG) of the active site branch (PG-1) of Radix Bupleuri Bupleurum chinense polysaccharide 2IIc was prepared, and the quality of Bupleurum chinense was investigated using this antibody. In vivo dynamics of active polysaccharides. It is speculated that the antigenic determinant of this antibody is the sugar side chain moiety that is sensitive to exo-β(1→3)-galactanase in the branch. Therefore, this time, various glycosidases were used to further analyze the branched micro-structure and the antibody’s antigenic determinants.