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作者研究了酪氨酸激酶抑制剂EGP57148B,选择性抑制BCR-ABL阳性细胞的生长作用。 材料与方法 单独或联合应用重组G-CSF、IL-3、促红细胞生成素,观察9例CML慢性期病人和3名正常人造血细胞CFU-GM和BFU-E集落形成,观察在梯度浓度CGP57148B中的细胞集落形成,其中部分标本加入1.0μmol/L CGP57148B后检测集落细胞BCR-ABL表达情况。从外周血或新鲜骨髓中分离出MNCs,部分标本再分离CD34~+细胞,MNCs作甲基纤维素平板培养,镜下计数第7天、第14天CFU-GM集落数目及第14天BFU-E
The authors studied the tyrosine kinase inhibitor EGP57148B, which selectively inhibits the growth of BCR-ABL positive cells. MATERIALS AND METHODS Recombinant G-CSF, IL-3 and erythropoietin were applied alone or in combination. The CFU-GM and BFU-E colonies were observed in 9 CML chronic phase patients and 3 normal human hematopoietic cells, and the results were observed in the gradient concentration CGP57148B Of the colony formation, some of which specimens after adding 1.0μmol / L CGP57148B detection of colony cells BCR-ABL expression. MNCs were isolated from peripheral blood or fresh bone marrow, CD34 + cells were isolated from some of the samples, and MNCs were cultured in methylcellulose. The number of CFU-GM colonies on day 7 and 14 and the number of BFU- E