放射受体实验(RRA)的方法学选择合适的标记配体标记配体应该具有非标记配体同样的化学性质,即同位素标记并不改变对受体的专一性.其次,为了保证最大灵敏度和减少非专一结合,标记配体必须具有高比活性.非专一结合是指标记配体与非受体部位的不可逆结合. Radiological receptor assay (RRA) methodology selection Appropriate labeled ligands Ligands should have the same chemistry of non-labeled ligands, ie, isotope labeling does not alter the specificity of the receptor Second, in order to ensure maximum sensitivity With reduced non-specific binding, the labeled ligand must have a high specific activity, and non-specific binding refers to the irreversible binding of labeled and non-receptor sites.