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AIM:To investigate the effect of acupuncture and moxibustionon epithelial cell apoptosis and expression of Bcl-2,Bax,fasand FasL proteins in rat ulcerative colitis.METHODS:A rat model of ulcerative colitis was estabelishedby immunological methods and local stimulation.All ratswere randomly divided into model control group (MC),electro-acupuncture group (EA),herbs-partition moxibustiongroup (HPM).Normal rats were used as normal controlgroup (NC).Epithelial cell apoptosis and expression ofBcl-2,Bax,fas and FasL proteins were detected by TUNELand immunohistochemiscal method respectively.RESULTS:The number of epithelial cell apoptosis in MCwas significantly higher than that in NC,and was markedlydecreased after the treatment with herbs-partitionmoxibustion or electro-acupuncture.The expression of Bcl-2,Bax,fas and FasL in colonic epithelial cells in MC was higherthan that in NC,and was markedly down-regulated by herbs-partition moxibustion or electro-acupuncture treatment.CONCLUSION:The pathogenesis of ulcerative colitis in ratsinvolves abnormality of apoptosis.Acupuncture andmoxibustion can regulate the expression of Bcl-2,Bax,fasand FasL proteins and inhibit the apoptosis of epithelial cellsof ulcerative colitis in rats by Bcl-2/Bax,fas/FasL pathways.
AIM: To investigate the effect of acupuncture and moxibustionon epithelial cell apoptosis and expression of Bcl-2, Bax, fasand FasL proteins in rat ulcerative colitis. METHODS: A rat model of ulcerative colitis was estabelishedby immunological methods and local stimulation. All rats were randomly selected divided into model control group (MC), electro-acupuncture group (EA), herbs-partition moxibustiongroup (HPM). Normal rats were used as normal controlgroup (NC) .Epithelial cell apoptosis and expression of Bcl-2, Bax, fas and FasL proteins were detected by TUNEL and immunohistochemiscal method respectively .RESULTS: The number of epithelial cell apoptosis in MCwas significantly higher than that in NC, and was markedly created after the treatment with herbs-partitionmoxibustion or electro-acupuncture. The expression of Bcl-2, Bax, fas and FasL in colonic epithelial cells in MC was higherthan that in NC, and was markedly down-regulated by herbs-partition moxibustion or electro-acupuncture treatment .CONCLUSION: The pa thogenesis of ulcerative colitis in rats in volves abnormality of apoptosis. Acupuncture and moxibustion can regulate the expression of Bcl-2, Bax, fasand FasL proteins and inhibit the apoptosis of epithelial cells of ulcerative colitis in rats by Bcl-2 / Bax, fas / FasL pathways.