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采用ＰＣＲ方法特异性扩增恶性疟原虫云南株（ＰＦＤ－３／ＹＮ）富含组氨酸蛋白Ⅱ基因，经基因序列测定后克隆于ｐＷＲ４５０－１融合蛋白表达载体，并转化大肠杆菌ＴＧ１。在不同菌体浓度及不同剂量ＩＰＴＧ诱导下检测ＨＲＰⅡ融合蛋白的表达。采用Ｄｏｔ－ＥＬＩＳＡ及Ｗｅｓｔｅｒｎ－ｂｌｏｔ分析对表达产物进行鉴定。结果显示ＨＲＰⅡ基因与ｐＷＲ４５０－１重组后在大肠杆菌ＴＧ１中表达一６８ＫＤａ的融合蛋白，当工程菌ＯＤ５９０为１．０～１．２时，加入终浓度１ｍｍｏｌ／ＬＩＰＴＧ进行诱导，表达量较高。Ｄｏｔ－ＥＬＩＳＡ和Ｗｅｓｔｅｒｎｂｌｏｔ分析表明ＨＲＰⅡ基因表达产物能被抗ＨＲＰⅡ单克隆抗体所识别
The genomic DNA of Plasmodium falciparum (PFD-3 / YN) -rich histidine II was amplified by PCR and cloned into pWR450-1 fusion protein expression vector and transformed into E.coli TG1. The expression of HRPII fusion protein was detected under different bacterial concentrations and different doses of IPTG. The expression products were identified by Dot-ELISA and Western-blot analysis. The results showed that the recombined HRPⅡ gene and pWR450-1 expressed a 68KDa fusion protein in E. coli TG1. When the OD590 of the recombinant strain was 1.0-1.2, the recombinant protein was induced with 1mmol / L IPTG and the expression level was high. Dot-ELISA and Western blot analysis showed that HRPII gene expression product was recognized by anti-HRPII monoclonal antibody