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目的 :探讨酶抑制剂乌司他丁 (ulinastatinforinjection ,UTI)对心肺脑复苏后肿瘤坏死因子 α(TNF α)、白细胞介素 6 (IL 6 )、白细胞介素 10 (IL 10 )和脑超微结构的影响。方法 :将 2 4只成年健康普通级SD大鼠 ,随机分成对照组、复苏组和乌司他丁组 ,每组 8只。对照组仅行气管切开术 ,不夹闭气管 ,不造成心跳呼吸骤停 ;复苏组与乌司他丁组建立心肺脑复苏模型 ,乌司他丁组在心肺脑复苏时加用单次UTI 10 6U·Kg-1。对照组在气管切开即刻、0 .5、2、4、6h采集血标本 ,复苏组与乌司他丁组分别在气管切开即刻、复苏成功后 0 .5、2、4、6h采集血标本 ,以放免法测定血清中TNF α、IL 6、IL 10的水平。实验结束时每组随机取 2只动物快速断头取皮层脑组织观察脑超微结构的变化。结果 :对照组中TNF α、IL 6、IL 10基本平稳 ,各时间点间无显著变化 (P >0 .0 5 )。复苏组复苏后TNF α、IL 6明显上升 ,IL 10明显下降 (P <0 .0 1或P <0 .0 5 )。乌司他丁组TNF α、IL 6、IL 10复苏后各时间点与气管切开即刻比较均无显著性差异 (P >0 .0 5 ) ,TNF α在复苏后0 .5、2、4h与复苏组同时间点比明显下降 (P <0 .0 5 ) ,IL 10在复苏后 0 .5、6h与复苏组同时间点比明显升高 (P <0 .0 5 )。脑组织超微结构改变 :乌司他丁?
Objective: To investigate the effect of enzyme inhibitor inhibitor ulinastatin for injection (UTI) on the expression of tumor necrosis factor α (TNF α), interleukin 6 (IL 6), interleukin 10 (IL 10) Effect of structure. Methods: Twenty-four adult healthy normal SD rats were randomly divided into control group, resuscitation group and ulinastatin group, with 8 rats in each group. The control group only tracheotomy, not tracheal closure, does not cause cardiac arrest and resuscitation; recovery group and ulinastatin group established cardiopulmonary and cerebral resuscitation model, ulinastatin group in cardiopulmonary resuscitation plus single UTI 10 6U · Kg-1. Blood samples were collected at 0.5, 2, 4 and 6 hours immediately after tracheotomy in the control group, and blood was collected at 0, 5, 2, 4 and 6 hours after the tracheotomy was performed in the resuscitation group and the ulinastatin group Specimens were determined by radioimmunoassay serum TNF α, IL 6, IL 10 levels. At the end of the experiment, two animals in each group were randomly selected to observe the ultrastructure of the brain by rapid decapitation. Results: The levels of TNFα, IL-6 and IL-10 in the control group were basically stable with no significant changes at all time points (P> 0.05). After resuscitation, the levels of TNFα and IL-6 in the resuscitation group increased significantly, and IL-10 decreased significantly (P <0.01 or P <0.05). The levels of TNFα, IL-6 and IL-10 in the ulinastatin group were not significantly different from those after tracheotomy at each time point (P> 0.05) (P <0.05) at the same time point compared with the resuscitation group (P <0.05), IL 10 at the same time point of resuscitation was significantly higher than that of the resuscitation group (P <0.05). Ultrastructural changes in brain tissue: ulinastatin?