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利用8种不同浓度的甲基磺酸乙酯(EMS),4种不同的浸种时间,处理甜瓜品系“3-2-2”种子,根据M1植株发芽率,筛选出适宜的诱变剂量为浓度1.2%,诱变时间24 h。采用这种方法构建了含有67个M1家系及相应自交M2种子的甜瓜突变体库。对M1群体541个单株的表型变异进行了全生育期初步调查,总的表型变异频率达71.53%。对M2群体603个单株的节间长度、主蔓粗度、叶片长度、叶片宽度、叶柄长度、叶柄粗度共6个表型性状进行形态学调查表明:除节间长度外,其他5个性状和野生型相比均差异显著;不同性状的变异系数不同,叶柄长度的变异系数最大,达15.37%,节间长度的变异系数最小,为7.10%;在M2中,编号为25的家系叶片出现了黄色和绿色的性状分离。
8 kinds of different concentrations of ethyl methanesulfonate (EMS), 4 different soaking time, the treatment of melon lines “3-2-2 ” seeds, according to the germination rate of M1 plants, screened the appropriate mutagenic dose The concentration of 1.2%, mutagenesis time 24 h. In this way, a library of melon mutants containing 67 M1 families and corresponding selfing M2 seeds was constructed. The phenotypic variation of 541 individuals in the M1 population was initially investigated during the whole growth period, with a total phenotypic frequency of 71.53%. Sixty-three phenotypic traits of internode length, main vine roughness, leaf length, leaf width, petiole length and petiole thickness of 603 individuals in M2 population were investigated by morphological investigation. The results showed that in addition to internode length, the other 5 The coefficient of variation of different traits was different, the coefficient of variation of petiole length was the largest, reaching 15.37%, and the coefficient of variation of internode length was the smallest, which was 7.10%. In M2, the number of family leaves A separation of yellow and green characters occurred.