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探讨大鼠谷胱甘肽S-转移酶P1基因上游增强子元件及作用于其上特异性结合蛋白与该基因在癌细胞中高表达的关系,用荧光素酶报告系统在HeLa及CBRH7919细胞中确定了增强子元件Ⅰ(GPEⅠ)及增强子元件Ⅱ(GPEⅡ)的活性,并将GPEⅡ的增强子活性部位定位于其上游84bp的GPEⅡ-1区域内。分析对比不同细胞中结合于GPEⅠ核心序列(cGPEⅠ)、GPEⅡ-1上特异性核结合蛋白,发现HeLa,CBRH7919细胞中存在的cGPEⅠ特异性结合蛋白及GPEⅡ-1特异性的64ku结合蛋白在正常大鼠肝细胞中则不存在。因此,上述反式作用因子可能与GPEⅠ,GPEⅡ的增强子活性及该基因在癌细胞中高表达密切相关。
To investigate the relationship between the upstream enhancer element of rat glutathione S-transferase P1 gene and its specific binding protein and the high expression of this gene in cancer cells, and to determine it in HeLa and CBRH7919 cells with the luciferase reporter system. The activities of enhancer element I (GPEI) and enhancer element II (GPEII) were enhanced, and the active site of the enhancer of GPEII was located within the 84 bp region of GPEII-1 upstream of it. Analysis and comparison of different cell binding to GPEI core sequence (cGPEI), GPEII-1 specific nuclear binding protein, found that HeLa, CBREI specific binding protein in CBRH7919 cells and GPEII-1 specific 64ku binding protein in normal large Rat liver cells do not exist. Therefore, the above-mentioned trans-acting factors may be closely related to GPEI, enhancer activity of GPEII and high expression of the gene in cancer cells.