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目的:探讨刺五加有效部对MPP+诱导的PC12细胞损伤的保护作用。方法:以不同浓度、不同作用时间的MPP+作用于PC12细胞,MTT法检测细胞存活率,考察其量效关系和时效关系。用不同浓度的刺五加有效部位处理后,与MPP+共同孵育,MTT法检测细胞存活率,流式细胞仪测凋亡率,紫外-可见分光光度计测LDH,NO,NOS,MDA含量,考察刺五加有效部位的保护作用。结果:刺五加有效部位终浓度达到200,400mg·mL-1时,可明显提高PC12细胞存活率,抑制细胞凋亡率,降低LDH,NO,NOS,MDA的含量,有统计学意义(P<0.05)。结论:刺五加有效部位对MPP+诱导的PC12细胞损伤具有一定的保护作用,其机制可能是通过减少LDH漏出,降低NO,NOS,MDA的含量,从而抑制细胞凋亡,提高细胞存活率而实现的。
Objective: To investigate the protective effect of Acanthopanax senticosus effective fraction on MPP + -induced PC12 cell injury. Methods: PC12 cells were treated with MPP + at different concentrations and different time. Cell viability was detected by MTT assay. The relationship between dose-effect and time-effect was observed. After treated with different concentrations of Acanthopanax senticosus, the cells were incubated with MPP +, the cell viability was measured by MTT assay, the apoptosis rate was measured by flow cytometry, and the content of LDH, NO, NOS and MDA were measured by MTT assay Acanthopanax effective part of the protective effect. Results: After the effective concentration of Acanthopanax senticosus reached 200,400 mg · mL-1, the survival rate of PC12 cells was significantly decreased, the apoptosis rate of PC12 cells was decreased, and the levels of LDH, NO, NOS and MDA were decreased (P < 0.05). Conclusion: The effective site of Acanthopanax senticosus could protect MPI + -induced injury of PC12 cells by decreasing the leakage of LDH, decreasing the content of NO, NOS and MDA, and thus inhibiting the apoptosis and increasing the cell survival rate of.