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目的:建立藤黄药材的高效液相指纹图谱方法。方法:采用Luna C8(4.6 mm×250 mm,5μm)色谱柱,柱温25℃,以乙腈-0.1%冰乙酸水溶液为流动相梯度洗脱,检测波长362 nm,流速为1.0 mL·min-1。采用国家药典委员会出版的《中药色谱指纹图谱相似度评价》(2004年A版)软件,对11批不同批次的藤黄药材指纹图谱进行相似度计算。结果:各批藤黄药材中有13个共有峰,各峰分离度良好,各批次藤黄药材间共有峰的相对保留时间RSD均<1.0%,药材间相似度均>90%。结论:本方法具有良好的精密度、重复性、稳定性,各共有峰间分离度高,可用于藤黄药材的真伪鉴别和质量综合评价。
Objective: To establish a HPLC fingerprint of Garcinia cambogiae. Methods: The mobile phase was eluted with acetonitrile-0.1% glacial acetic acid at a column temperature of 25 ℃ on a Luna C8 (4.6 mm × 250 mm, 5 μm) column with a detection wavelength of 362 nm and a flow rate of 1.0 mL · min -1 . The similarity of fingerprints of 11 batches of Garcinia cambogia was calculated using the “Evaluation of the Similarity of Chromatographic Fingerprints of Traditional Chinese Medicines” (Version A, 2004) published by the State Pharmacopoeia Commission. Results: There were 13 common peaks in each batch of Garcinia rhynchophylla and the peak separation was good. The relative retention times (RSDs) of the common peaks in each batch of Garcinia rhynchophylla were less than 1.0%, and the similarities among the medicinal materials were both> 90%. Conclusion: The method has good precision, repeatability, stability, high separation between the common peak, which can be used for the authenticity identification and quality comprehensive evaluation of Garcinia cambogiae.