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目的研究OX40-OX40L轴的激活对大鼠血管平滑肌细胞(VSMC)增殖和迁移能力的影响。方法原代培养大鼠主动脉VSMC并传代,培养液中添加OX40L(0、0.1、1、10或20mg/L)刺激24h,或在培养基中加入选定浓度OX40L10mg/L,刺激6、12、24、36、48h。以CCK-8法测定VSMC增殖率,以划痕法测定VSMC迁移率。结果在OX40L浓度从0～20mg/L范围内,刺激24h后,VSMC的增殖、迁移能力的增强与OX40L浓度呈剂量依赖性,并明显大于OX40L0mg/L组(P<0.05)。OX40L浓度达到10mg/L时,VSMC的增殖、迁移能力已接近最大值。应用10mg/L的OX40L刺激不同时间(6、12、24、36、48h)后,发现随着刺激时间的延长,VSMC增殖、迁移能力逐渐增强;刺激至24h,VSMC增殖能力达到最大值。结论 OX40-OX40L轴的激活可能通过促进VSMC的增殖及迁移影响动脉粥样斑块的进展。
Objective To investigate the effects of OX40-OX40L axis activation on proliferation and migration of rat vascular smooth muscle cells (VSMCs). Methods VSMCs of primary aorta were cultured and passaged in primary culture. OX40L (0, 0.1, 1, 10 or 20 mg / L) was added into the culture medium for 24 h or OX40L 10 mg / L was added to the culture medium to stimulate 6,12 , 24,36,48h. The proliferation rate of VSMC was determined by CCK-8 method and the mobility of VSMC was determined by scratch method. Results After stimulation with OX40L for 24 h, the proliferation and migration of VSMC increased in a dose - dependent manner and significantly higher than that of OX40L0 mg / L group (P <0.05). OX40L concentration of 10mg / L, VSMC proliferation and migration ability is close to the maximum. After stimulated with 10mg / L OX40L for different times (6, 12, 24, 36, 48h), VSMC proliferated and migrated gradually with the prolongation of stimulation time. The proliferative ability of VSMC reached its maximum at 24h. Conclusion The activation of OX40-OX40L axis may affect the progression of atherosclerotic plaque by promoting VSMC proliferation and migration.