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薄层色谱法有分离能力强、效果明显等特点,已被广泛地应用于农药残留量的定性、定量分析。鉴于现有的薄层酶法,由于酶源的制备,以及其他条件因素的限制,不能广泛普遍地应用。薄层化学法的灵敏度又不够高。现我们研究了一种简便、快速、灵敏测定食用作物上甲胺磷残留量的改良薄层法,特别适用于检样多、含量低、杂质多的情况。本测定法将样品提取液不经柱层析,直接点样于改良薄层板(150微米厚度)上,用不同溶剂以两次
Thin-layer chromatography has the ability of separation, the effect is obvious, has been widely used in qualitative and quantitative analysis of pesticide residues. In view of the existing thin-layer enzymatic method, it can not be widely used due to the preparation of the enzyme source and other conditions. The sensitivity of thin-layer chemistry is not high enough. We have now studied a simple, rapid and sensitive method for the determination of methamidophos residues in edible crops using an improved thin-layer method that is particularly suitable for the detection of large amounts of samples with low levels of impurities and impurities. This assay samples the extract without column chromatography, directly spotted on a modified plate (150 microns thickness), with different solvents in two