目的通过建立肺炎支原体(MP)感染BALB/c小鼠的模型,探讨MP感染后小鼠支气管肺泡灌洗液(BALF)中炎性因子(IFN-γ和IL-4、IL-5和IL-10)的含量变化及其对气道阻力的影响。方法 50只BALB/c小鼠随机分为MP感染组(MP组)和PBS对照组(N组),每组25只,分别于接种后第3d、7d、14d、21d和30d从各组中取5只鼠,取肺脏制作病理切片,HE染色观察病理组织学变化;RT-PCR对模型进行鉴定;ELISA试剂盒检测BALF中IFN-γ、IL-4、IL-5和IL-10含量;同时用肺功能仪测定各组小鼠肺的吸气阻力、呼气阻力和顺应性。结果 MP感染小鼠后IFN-γ、L-4、IL-5和IL-10的含量较N组明显升高(<0.05);吸气阻力、呼气阻力较N组增加(<0.05),顺应性降低较N组增加(<0.05),第7天时达高峰,随后下降30天恢复正常。结论 MP感染BALB/c小鼠后气道阻力增加、顺应性降低,炎性因子过表达。 Objective To establish a model of Mycoplasma pneumoniae (MP) infection in BALB / c mice to explore the role of inflammatory cytokines (IL-4, IL-5 and IL-4) in bronchoalveolar lavage fluid (BALF) 10) and its effect on airway resistance. Methods Fifty BALB / c mice were randomly divided into MP infection group (MP group) and PBS control group (N group), with 25 rats in each group. The BALB / c mice were divided into three groups at the 3rd, 7th, 14th, 21st and 30th day after inoculation The pathological changes were observed by HE staining; the model was identified by RT-PCR; the contents of IFN-γ, IL-4, IL-5 and IL-10 in BALF were detected by ELISA kit; At the same time, lung function test was used to measure the inspiratory resistance, expiratory resistance and compliance of the lungs in each group. Results The levels of IFN-γ, L-4, IL-5 and IL-10 in MP-infected mice were significantly higher than those in N group (P <0.05) Compliance decreased more than the N group (<0.05), peaked on the 7th day, and then returned to normal after 30 days of decline. Conclusion MP infection in BALB / c mice increased airway resistance, decreased compliance, and over-expression of inflammatory cytokines.