论文部分内容阅读
抗组胺药敏感性细胞色素Ｐ－４５０（简称ＨＰ－４５０）主要存在于肝脏微粒体，是一个有组胺Ｈ１受体和细胞色素Ｐ－４５０（ＣｙｔＰ－４５０）两种物质特性的蛋白质，我们利用［３Ｈ］ｍｅｐｙｒａｍｉｎｅ为同位素标记进行受体配体结合实验，检测ＨＰ－４５０随着大鼠增龄而致的动力学特征，同时又从酶学比较Ｃｙｔｐ－４５０在不同年龄组大鼠肝脏微粒体内的变化规律和趋势，发现ＨＰ－４５０和ＣｙｔＰ－４５０都明显地随着大鼠年龄而变化，其中ＨＰ－４５０的Ｂｍａｘ值和Ｋｄ值在大鼠出生后２至８周内增加最快，８至１０周达到最高水平并趋向稳定状态，ＣｙｔＰ－４５０也有同样的趋势，并且ＨＰ－４５０的增长与ＣｙｔＰ－４５０的增长幅度具有良好的相关性（相关系数ｒ＝０．６２５）。实验中未曾发现性别间的差异。
Anti-histamine drug sensitive cytochrome P-450 (HP-450) is mainly present in the liver microsome and is a protein that has histamine H1 receptors and cytochrome P-450 (CytP-450) We used [3H] mepyramine isotope labeling receptor ligand binding experiments to detect HP-450 with the kinetic characteristics of rats induced by aging, at the same time from the enzymatic comparison of Cytp-450 in different age groups of rat liver The results showed that HP-450 and CytP-450 obviously changed with the age of rats. The Bmax value and Kd value of HP-450 increased fastest in 2 to 8 weeks after birth , Reached the highest level at 8-10 weeks and reached a steady state. CytP-450 also had the same trend, and there was a good correlation between the growth of HP-450 and the growth rate of CytP-450 (correlation coefficient r = 0.625). No gender differences were found in the experiment.