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Objective:To investigate the influence of GST-π gene transfer on drug-resistance of human cord blood CD34 + cells.Methods:CD34 + cells were purified from cord blood from normal full-term pregnancy.Gene transduction into human cord blood CD34 + cells was carried out using GST-π gene containing retrovirus vector.The GST-π gene expression in transduced CD34 + cell was confirmed by RT-PCR.After confirmation of GST-π gene transfer,the transfected CD34 + cells were cultured by colony assay in the presence of carboplatin.Results:GST-π mRNA was detected in 30% of CFU-GM derived from GST-π gene transduced CD34 + cells.In vitro drug resistance test showed that the number of CFU-GM formed was significantly higher (2～3 fold) in GST-π gene transduced CD34 + cells than untransduced CD34 +cells.Conclusion:GST-π gene transfer can confer resistance to hematopoietic progenitors against carboplatin in vitro.
Objective: To investigate the influence of GST-π gene transfer on drug-resistance of human cord blood CD34 + cells. Methods: CD34 + cells were purified from cord blood from normal full-term pregnancy. Gene transduction into human cord blood CD34 + cells was carried out using GST-π gene containing a retrovirus vector. The GST-π gene expression in transduced CD34 + cell was confirmed by RT-PCR. After confirmation of GST-π gene transfer, the transfected CD34 + cells were cultured by colony assay in the presence of carboplatin. Results: GST-π mRNA was detected in 30% of CFU-GM derived from GST-π gene transduced CD34 + cells. In vitro drug resistance test showed that the number of CFU-GM formed was significantly higher ~ 3 fold) in GST-π gene transduced CD34 + cells than untransduced CD34 + cells. Confluence: GST-π gene transfer can confer resistance to hematopoietic progenitors against carboplatin in vitro.