前列腺素和白细胞三烯是花生四烯酸分别经环氧酶和脂氧酶合成的产物。这两类物质是炎症和过敏反应过程中极重要的介质。国外对抗炎药物和抗过敏药物对这两类物质的影响的研究很重视。但国内这方面的报道很少。本文报道托美汀、汉防己甲素、噻呱酮对前列腺素E_2与白细胞三烯B_4生物合成的影响。材料与方法花生四烯酸,PGE_2,PGB_2,为Sigma公司产品,白细胞三烯B_4(leukotriene B_4,LTB_4)为加拿大J.Rokach教授赠送。PGE_2含量的测定方法如文献1。环氧酶粗制品粉末溶解于Tris-HCl缓冲液中, Prostaglandins and leukotrienes are the products of arachidonic acid synthesized by cyclooxygenase and lipoxygenase, respectively. These two classes of substances are extremely important mediators of inflammation and anaphylaxis. Foreign studies on the effects of anti-inflammatory drugs and anti-allergic drugs on these two substances are very important. However, there are few reports in this area in China. This article reports the effects of tolmetin, tetrandrine and thidulin on prostaglandin E 2 and leukotriene B 4 biosynthesis. MATERIALS AND METHODS Arachidonic acid, PGE_2 and PGB_2 were purchased from Sigma Company and leukotriene B_4 (LTB_4) from Canada as Professor J. Rokach. The determination of PGE_2 content as literature 1. Crude cyclooxygenase powder was dissolved in Tris-HCl buffer,