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【目的】研究细菌作用下碳酸盐矿物的形成过程有助于了解微生物成矿的机理。【方法】在LagoaVermelha培养基中(Mg/Ca为6:1)对一株分离自土壤样品的梭菌MH18菌株进行了为期35天的碳酸盐矿物培养实验,同时还完成了一组无菌对照实验。利用X-射线衍射技术对沉淀物的矿物成分进行了测定,利用光学显微镜和扫描电子显微镜对沉淀物的形态进行了系统的观察。【结果】MH18菌株在Lagoa Vermelha培养基中诱导形成了以高镁方解石为主的碳酸盐矿物;这些矿物起初具有哑铃状的外形,后来发展为球状;无菌对照实验中出现少量沉淀物,但X-射线衍射技术图谱显示它们是非晶态物质。【结论】MH18菌株具有促进碳酸盐矿物结晶的功能;碳酸盐矿物的特殊形态(哑铃状和球状)可能与细菌形态存在着某种成因上的联系。
【Objective】 The study of the formation of carbonate minerals under the action of bacteria helps to understand the mechanism of microbial mineralization. 【Method】 A 35-day carbonate mineral culture experiment was carried out on a Clostridium strain MH18 isolated from soil samples in Lagoa Vermelha medium (Mg / Ca 6: 1), and a group of sterile Control experiment. The mineral components of the precipitate were determined by X-ray diffraction. The morphology of the precipitate was systematically observed by light microscopy and scanning electron microscopy. 【Result】 MH18 strains induced the formation of carbonate minerals dominated by high-Mg calcite in Lagoa Vermelha medium. These minerals initially had a dumbbell-like appearance and later developed into globular forms. A small amount of sediment appeared in the sterile control experiments, However, X-ray diffraction patterns show that they are amorphous. 【Conclusion】 The MH18 strain has the function of promoting the crystallization of carbonate minerals. The special morphology of carbonate minerals (dumbbell shape and globular shape) may have some causal relationship with the bacterial morphology.