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目的:探究波棱瓜子中活性成分乙酸乙酯提取物(HE)与脂肪油(HSO)配伍对刀豆蛋白A(Con A)诱导的免疫性肝损伤小鼠的保护作用及其机制。方法:将小鼠80只随机区组分为8组,即空白组,模型组,联苯双酯组(阳性组),HSO组,HE组,组分配伍1∶1,1∶2,2∶1组,每组10只。治疗组分别灌胃(ig)给予波棱瓜子HE,HSO及二者不同剂量配伍药液连续8 d,采用尾静脉一次性注射Con A 30 mg·kg-1诱导造成小鼠免疫性肝损伤模型。造模8 h后采血,制备肝匀浆。计算小鼠的肝脾系数;酶联免疫法(ELISA)检测小鼠血清谷丙转氨酶(ALT),谷草转氨酶(AST),一氧化氮(NO)及肝脏匀浆中超氧化物歧化酶(SOD),丙二醛(MDA)等生化指标的活性及含量;同时对肝组织进行病理学检查。结果:波棱瓜子各治疗组均可降低免疫性肝损伤小鼠的肝脾脏器指数;降低小鼠肝损伤血清ALT,AST水平及NO的含量;增强肝匀浆中SOD活性,降低MDA的含量;减轻Con A对肝组织的病理损伤。其中有效组分HSO-HE 2∶1配伍组各指标较模型组均有显著性差异,护肝作用最佳。结论:波棱瓜子HE与HSO配伍护肝作用优于单一组分,提示配伍时具有协同增效的作用,其保肝机制可能与其抗炎、抗氧化以及清除自由基有关。
Objective: To investigate the protective effect and its mechanism of compatibility of ethyl acetate extract (HE) and fatty oil (HSO) active ingredient in melon seeds on immune-induced liver injury induced by Con A in mice. Methods: 80 mice were randomly divided into 8 groups: blank group, model group, bifendate group (positive group), HSO group, HE group, the components compatibility 1: 1, 1: : 1 group, 10 in each group. The rats in the treatment group were given gavage seeds of HE, HSO, and their different dosages for 8 consecutive days. The mice were immunized with Con A (30 mg · kg-1) . Blood samples were taken after 8 h of modeling to prepare liver homogenates. The hepatic and splenic indexes of mice were calculated. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), nitric oxide (NO) and liver homogenate superoxide dismutase (SOD) were detected by enzyme-linked immunosorbent assay , Malondialdehyde (MDA) and other biochemical indicators of activity and content; the same time, liver biopsy. Results: The treatment group of Bailuagua could reduce the indices of liver and spleen of mice with immune liver injury, reduce the levels of serum ALT, AST and the level of NO in mice with liver injury, increase the activity of SOD in liver homogenate and decrease the content of MDA ; Reduce Con A pathological damage to liver tissue. The effective components HSO-HE 2: 1 compatibility group than the model group indicators were significantly different, the best role in liver protection. Conclusion: The effect of HE and HSO combined with Heparin on the protection of liver is better than that of single component, which suggests that synergistic synergism may play a role in the protection of liver. The mechanism of protecting liver may be related to its anti-inflammatory, anti-oxidation and free radical scavenging.