β淀粉样蛋白(β-amyloid peptide,Aβ)是构成老年斑的主要成分,Aβ在阿尔茨海默病(Alzheimer’s disease,AD)的发生、发展中起主要作用。文章采用紫外和荧光光谱法研究人参皂苷Rb1与Aβ_(1-42)的相互作用,为AD药物筛选提供理论依据。研究表明,人参皂苷Rb1可以增强Aβ_(1-42)的荧光,二者发生相互作用,形成了络合物。人参皂苷Rb1与Aβ络合物的双倒数曲线呈良好的线性关系,说明人参皂苷Rb1与Aβ形成1∶1的络合物,络合常数约为214.5L·mol~(-1)·s~(-1)。 β-amyloid peptide (Aβ) is the main component of senile plaques. Aβ plays a major role in the development and progression of Alzheimer’s disease (AD). In this paper, the interaction between ginsenoside Rb1 and Aβ_ (1-42) was studied by ultraviolet and fluorescence spectroscopy, providing a theoretical basis for AD drug screening. Studies have shown that ginsenoside Rb1 can enhance the fluorescence of Aβ_ (1-42), the two interact to form a complex. Ginsenoside Rb1 and Aβ complex double reciprocal curve showed a good linear relationship, indicating that ginsenoside Rb1 and Aβ form a 1: 1 complex, the complex constant of about 214.5L · mol ~ (-1) · s ~ (-1).