论文部分内容阅读
目的通过纸片扩散(KB)法、最小抑菌浓度检测试纸条(MTS)及Vitek 2 Compact系统检测临床常见多重耐药菌对抗菌药物替加环素的体外敏感性,从而评价3种体外药物敏感试验检测的准确性。方法回顾性收集2014年1月-2015年3月分离的140株临床常见的多重耐药菌(不含铜绿假单胞菌),其中非发酵菌鲍曼不动杆菌119株,肠杆菌科21株。采用KB法、Vitek 2法和MTS法检测替加环素的最小抑菌浓度值,并将Vitek 2法和KB法的结果与MTS法进行比较。结果 MTS法、KB法和Vitek 2法检测119株鲍曼不动杆菌对替加环素敏感率分别为90.8%、88.2%、85.7%,差异无统计学意义(P>0.05);检测21株肠杆菌科菌对替加环素敏感率分别为81.0%、76.2%、81.0%,差异无统计学意义(P>0.05)。结论替加环素对铜绿假单胞菌以外的临床常见多重耐药菌具有较好的体外抗菌活性,但是不同的检测方法敏感率有所不同,均存在差异。对于鲍曼不动杆菌,KB法优于Vitek 2法;对于肠杆菌科菌,Vitek 2法检测结果优于KB法。
OBJECTIVE: To evaluate in vitro susceptibility of antimicrobial tigecycline to common multi-drug resistant bacteria in vitro by KB test, MTS and Vitek 2 Compact system. Accuracy of drug sensitivity test. Methods Retrospectively collected 140 clinically common multidrug-resistant bacteria (excluding Pseudomonas aeruginosa) isolated from January 2014 to March 2015. Among them 119 non-fermentative bacteria Acinetobacter baumannii, Enterobacteriaceae 21 Strain. KB method, Vitek 2 method and MTS method were used to test the minimum inhibitory concentrations of tigecycline. The results of Vitek 2 method and KB method were compared with MTS method. Results The sensitivity of 119 strains of Acinetobacter baumannii to MTS, KB and Vitek 2 assays were 90.8%, 88.2% and 85.7%, respectively, with no significant difference (P> 0.05) The sensitivity of Enterobacteriaceae to tigecycline was 81.0%, 76.2% and 81.0%, respectively, with no significant difference (P> 0.05). Conclusion Tigecycline has good in vitro antibacterial activity against clinical multi-drug resistant strains of Pseudomonas aeruginosa, but the sensitivity of different test methods is different, there are differences. For Acinetobacter baumanii, KB method is superior to Vitek 2 method; for Enterobacteriaceae, Vitek 2 method is superior to KB method.