论文部分内容阅读
本文将牛血清白蛋白(BSA)与钙调蛋白(Calmodulin,CaM)交联作为抗原,制备抗体,运用酶联免疫吸附法(ELISA)建立了测定CaM含量的标准曲线,通过EL ISA和流式细胞光度计观察了CaM拮抗剂0—(1—ethoxyl—bUtyl)berbarmine(EBB)对培养的成纤维细胞的CaM和DNA的影响,探讨了EBB的作用机理。
In this paper, bovine serum albumin (BSA) and calmodulin (CaM) were cross-linked as antigen to prepare antibody. The standard curve of CaM content was established by ELISA. The effect of CaM antagonist 0- (1-ethoxyl-bUtyl) berbarmine (EBB) on CaM and DNA of cultured fibroblasts was observed by cell photometer, and the mechanism of action of EBB was discussed.