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目的：为了合成金属螯合四肽并制备其单克隆抗体。方法：从初始原料起，采用液相接肽等方法，合成三乙撑四胺钴（Ⅲ）－Ｎ－（α－羧基－苯乙基）－β－Ａｌａ－Ｐｈｅ－β－Ａｌａ－ＧｌｙＯＨ半抗原，并将其偶联到载体蛋白ＢＳＡ上制成抗原，免疫小鼠，采用杂交瘤方法制备单克隆抗体；结果：成功地合成了该金属螯合四肽，获得了８株单克隆抗体杂交瘤细胞系，并对这８株ＭｃＡｂ类与亚类等特性进行了鉴定，结论：可根据需要化学合成一些分子量很小的肽，并制备其单克隆抗体。
Aim: To synthesize metal-chelated tetrapeptides and prepare their monoclonal antibodies. Methods: Starting from the starting materials, the liquid phase peptide and other methods were used to synthesize the cobalt (Ⅲ) -N- (α-carboxy-phenethyl) -β-Ala-Phe-β-Ala-GlyOH Antigens were prepared and conjugated to the carrier protein BSA to make antigens. The mice were immunized and the monoclonal antibodies were prepared by the hybridoma method. Results: The metal chelated tetrapeptides were successfully synthesized and 8 monoclonal antibodies were obtained Tumor cell lines, and the 8 strains of McAb and subclass identified such characteristics, Conclusion: According to the need for chemical synthesis of some small molecular weight peptides and preparation of their monoclonal antibodies.