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前言利用噬菌体診断痢疾的方法,过去只限于分离噬菌体或对分离出来的菌株用已知噬菌体进行鑑定。前者結果并不能說明同时有相应的致病菌存在;后者則受分离培养条件的限制,只能作輔助方法,仍不能滿足实际需要。 1956年和氏提出了噬菌体增殖反应。其理論基础是:特异性噬菌体与相应菌株相遇时,經过吸附及于菌体内潛育生长过程,最后噬菌体大量增殖。根据已知噬菌体的量的变化判断相应菌株的存在。反应程序如下: 1.于被检驗材料中严格定量地加入指示噬菌体。
Preface The use of bacteriophages for the diagnosis of dysentery has in the past been limited to the isolation of phage or the identification of isolated phages using known phages. The former results do not indicate the existence of the corresponding pathogens at the same time. The latter is limited by the conditions of isolation and culture and can only be used as auxiliary methods and still can not meet the actual needs. In 1956 and the phage proliferation reaction. The theoretical basis is: Specific phages and the corresponding strains met, after adsorption and growth in the bacterial growth process, and finally a large number of phage proliferation. The presence of the corresponding strain is judged on the basis of the change in the amount of the known phage. The reaction procedure is as follows: 1. The indicator phage is added strictly and quantitatively to the test material.