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用微电极细胞内记录小终板电位（ＭＥＰＰ）的电生理学技术，在６６只雌性、６２只雄性成年蟾蜍缝匠肌神经－肌接头研究了ＭＥＰＰ的季节性变化与年节律。结果：①１年里ＭＥＰＰ的逐月平均振幅呈现明显的季节性变化，在春季的３、４月最高，而在秋季的９、１０月最低；②单余弦法分析和零振幅检验证明，此季节性变化呈近年节律性波动（Ｐ＜０．００１），其调整中值、振幅和峰值时相３个参数在雌性分别为０．２９±０．０５ｍＶ、０．２５ｍＶ和－９７．１７°，在雄性分别为０．１９±０．０２ｍＶ、０．１６ｍＶ和－９２．０１°。雌性的年节律较雄性的更明显（Ｐ＜０．０５）；③无论雌、雄蟾蜍ＭＥＰＰ的频率波动在统计学上既无明显的季节性改变，也无年节律。
The electrophysiological techniques of microelectrode recording small endplate potential (MEPP) were used to study the seasonal changes and annual rhythm of MEPP in 66 female and 62 male adult toad sartorius muscle-muscle connectors. Results: ① The monthly average amplitude of MEPP showed a seasonal change in 1 year, which was the highest in March and April in spring and the lowest in September and October in autumn. ② The single-cosine method and zero-amplitude test showed that this season The changes of rhythms were rhythmic fluctuations in recent years (P <0.001). The three parameters of adjusting median, amplitude and peak phase were 0.29 ± 0.05mV, 0.25mV and -97.17 ° in female, In males were 0.19 ± 0.02 mV, 0.16 mV and -92.01 °, respectively. The annual rhythm of female was more obvious than that of male (P <0.05). ③The frequency fluctuation of MEPP in male and female toad was neither statistically significant nor seasonal.