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从用免疫学方法查出人绒毛膜促性腺激素(hCG)以来,发明了试管法血凝抑制试验和玻片法乳胶凝集抑制试验,但其敏感度在500～1,000IUhCG/L。第二代血凝抑制试验采用β-hCG 的单克隆扰血清,敏感度提高到250IUβ-hCG/L。本文评价用灵敏度为150IU hCG/Lβ-hCG 单克隆抗体血凝抑制试验多中心比较性试验结果。方法:在5个不同中心,将尿标本同时进行敏感度在150IU hCG/L的β-hCG 单克隆抗体试验与不同敏感度的 hCG 妊娠试验。共测定1,066份标本,包括40岁以上妇女的108份,已知妊娠妇女389份和根据病史明显非孕妇女569份。评价结果不一致的标本再用放免测定法(RIA)复查β-hCG。结果可见40岁以上妇女尿标本中1份 ETTM
Since the detection of human chorionic gonadotropin (hCG) by immunological methods, the in vitro hemagglutination inhibition test and the slide latex agglutination inhibition test were invented, but the sensitivity was 500 to 1,000 IUhCG / L. The second generation of hemagglutination inhibition test using β-hCG monoclonal interference sera, the sensitivity increased to 250IUβ-hCG / L. In this review, a multicenter comparative test was conducted using a hemagglutination inhibition test with a sensitivity of 150 IU hCG / Lβ-hCG monoclonal antibody. METHODS: Urine samples were simultaneously subjected to a β-hCG monoclonal antibody test with sensitivity of 150 IU hCG / L and hCG pregnancy tests of different sensitivities at five different centers. A total of 1,066 specimens were tested, including 108 women over the age of 40, 389 known pregnancies and 569 obviously nonpregnant women with a history of disease. Evaluation of inconsistent specimens and then radioimmunoassay (RIA) review of β-hCG. As a result, one ETTM was found in urine samples from women over the age of 40 years